Biospecimens, along with clinical and imaging data collected under the GARBH-Ini cohort from participants enrolled at ≤20 weeks of gestation and followed up during each trimester, at delivery, and postpartum.
temp Serum
Donor:Pregnant women
Timepoints:
(Period of gestation)		≤20 weeks,18-20 weeks,26-28 weeks, Delivery(Maternal & Cord blood) & 42 days to 6 months postpartum
Format:0.1-0.5 ml per cryovial frozen
Anticoagulant:Plain vacutainer tube
Storage:-80°C in deep freezer
temp EDTA Plasma
Donor:Pregnant women
Timepoints:
(Period of gestation)		≤20 weeks, 26-28 weeks, Delivery(Cord blood) & 42 days to 6 months postpartum
Format:0.3-0.5 ml per cryovial frozen
Anticoagulant:EDTA vacutainer tube
Storage:-80°C in deep freezer
temp DNA
Donor:Pregnant women
Timepoints:
(Period of gestation)		≤20 weeks, 18-20 weeks, 26-28 weeks, Delivery(Maternal & Cord blood) & 42 days to 6 months postpartum
Format:120 -125 μL per cryovial frozen
Anticoagulant:EDTA vacutainer tube
Storage:-80°C in deep freezer
temp Na citrate Plasma
Donor:Pregnant women
Timepoints:
(Period of gestation)		≤20 weeks,18-20 weeks,26-28 weeks, Delivery(Maternal & Cord blood) & 42 days to 6 months postpartum
Format:0.5 ml per cryovial frozen
Anticoagulant:Na citrate vacutainer tube
Storage:-80°C in deep freezer
temp PAXgene blood RNA tube
Donor:Pregnant women
Timepoints:
(Period of gestation)		≤20 weeks, 18-20 weeks, 26-28 weeks, Delivery & 42 days to 6 months postpartum
Format:2.0 ml per cryovial frozen
Anticoagulant:RNA PAXgene tube
Storage:-80°C in deep freezer
temp Buffy coat
Donor:Pregnant women
Timepoints:
(Period of gestation)		≤20 weeks, 26-28 weeks, Delivery(Cord blood) & 42 days to 6 months postpartum
Format:0.5 ml per cryovial frozen
Anticoagulant:EDTA vacutainer tube
Storage:-80°C in deep freezer
temp Urine/Urine pellet in RNA Later
Donor:Pregnant women
Timepoints:
(Period of gestation)		≤20 weeks, 18-20 weeks, 26-28 weeks & 42 days to 6 months postpartum
Format:0.5-1.5 ml per cryovial frozen
Anticoagulant:Sterile container
Storage:-80°C in deep freezer
temp Saliva
Donor:Pregnant women
Timepoints:
(Period of gestation)		≤20 weeks, 18-20 weeks, 26-28 weeks & 42 days to 6 months postpartum
Format:0.5-1.5 ml per cryovial frozen
Anticoagulant:Sterile container
Storage:-80°C in deep freezer
temp High vaginal swab sample
Donor:Pregnant women
Timepoints:
(Period of gestation)		≤20 weeks, 18-20 weeks, 26-28 weeks & 42 days to 6 months postpartum
Format:0.5-1.0 ml per cryovial frozen
Anticoagulant:N/A
Storage:-80°C in deep freezer
temp Whatman card
Donor:Pregnant women
Timepoints:
(Period of gestation)		≤20 weeks,18-20 weeks,26-28 weeks, Delivery(Maternal & Cord blood) & 42 days to 6 months postpartum
Format:3 drops
Anticoagulant:EDTA vacutainer tube
Storage:Room temperature
temp Placental punch biopsy
Donor:Pregnant women
Timepoint:
(Period of gestation)		At delivery
Format:8mm Punch in RNA Later, 10 mm punch in Flash Frozen & Diamond shape cut Formalin
Anticoagulant:RNA Later, Flash frozen & Formalin
Storage:-80°C in deep freezer/Room temperature
temp Umbilical cord tissue
Donor:Pregnant women
Timepoint:
(Period of gestation)		At Delivery
Format:8mm Punch in RNA Later, 10 mm punch in Flash Frozen & Diamond shape cut Formalin
Anticoagulant:RNA later, Flash Frozen & Formalin
Storage:-80°C in deep freezer/Room temperature
temp Placental membrane
Donor:Pregnant women
Timepoint:
(Period of gestation)		At Delivery
Format:1 x 3cm each
Anticoagulant:RNA later, Flash Frozen & Formalin
Storage:-80°C in deep freezer/Room temperature
temp 903 Protein saver card (Cord blood)
Donor:Pregnant women
Timepoint:
(Period of gestation)		At Delivery
Format:5 drops
Anticoagulant:EDTA vacutainer tube
Storage:-80°C in deep freezer
temp 903 Protein saver card (Neonatal heel prick blood)
Donor:Neonate
Timepoint:
(Period of gestation)		At Delivery (at birth)
Format:5 drops
Anticoagulant:N/A
Storage:-80°C in deep freezer